Journal: Molecular Medicine Reports

Article Title: Effects of bradykinin on TGF-β1-induced epithelial-mesenchymal transition in ARPE-19 cells

doi: 10.3892/mmr.2018.8556

Figure Lengend Snippet: Primers used for reverse transcription-quantitative polymerase chain reaction amplification.

Article Snippet: The antibodies and dilution ratios were as follows: Anti-E-cadherin (1:500; cat. no. GTX100443, GeneTex International Corporation, Hsinchu, Taiwan), mouse anti-α-SMA (1:500; cat. no. BS70000, Bioworld Technology, Inc., St. Louis Park, MN, USA), vimentin (cat. no. BS1491, 1:500; Bioworld Technology, Inc.), anti-pSmad3 (1:500; cat. no. BS4173, Bioworld Technology, Inc.), anti-Smad7 (1:500; cat. no. BS60366, Bioworld Technology, Inc.) and anti-GAPDH (1:500; cat. no. G9545, Sigma-Aldrich; Merck KGaA).

Techniques: Polymerase Chain Reaction, Amplification

Journal: Molecular Medicine Reports

Article Title: Effects of bradykinin on TGF-β1-induced epithelial-mesenchymal transition in ARPE-19 cells

doi: 10.3892/mmr.2018.8556

Figure Lengend Snippet: BK inhibits TGF-β1-induced epithelial-mesenchymal transition via the TGF/Smad signaling pathway. ARPE-19 cells were treated with BK, TGF-β1, BK plus TGF-β1, TGF-β1 plus HOE140, BK plus TGF-β1 plus HOE140 and Dulbecco's modified Eagle's medium for 48 h before detection. (A) Protein expression of pSmad3 and Smad7 was identified by western blot analysis. (B) Reverse transcription-quantitative polymerase chain reaction analysis and (C) immunofluorescence staining (magnification, ×200). **P<0.001 and *P<0.05. TGF-β1, transforming growth factor-β1; BK, bradykinin; pSmad, phosphorylated mothers against decapentaplegic homolog.

Article Snippet: The antibodies and dilution ratios were as follows: Anti-E-cadherin (1:500; cat. no. GTX100443, GeneTex International Corporation, Hsinchu, Taiwan), mouse anti-α-SMA (1:500; cat. no. BS70000, Bioworld Technology, Inc., St. Louis Park, MN, USA), vimentin (cat. no. BS1491, 1:500; Bioworld Technology, Inc.), anti-pSmad3 (1:500; cat. no. BS4173, Bioworld Technology, Inc.), anti-Smad7 (1:500; cat. no. BS60366, Bioworld Technology, Inc.) and anti-GAPDH (1:500; cat. no. G9545, Sigma-Aldrich; Merck KGaA).

Techniques: Modification, Expressing, Western Blot, Real-time Polymerase Chain Reaction, Immunofluorescence, Staining

Journal: Journal of Biological Chemistry

Article Title: Vascular Endothelial Cell Growth Factor Attenuates Actions of Transforming Growth Factor-β in Human Endothelial Cells

doi: 10.1074/jbc.m407423200

Figure Lengend Snippet: FIG. 7. Effect of VEGF on binding of Smad7 to TGF- receptor type I in HUVEC. HUVEC cells were incubated with 30 nM VEGF for 60 min, and the cell lysates were immunoprecipitated (IP) by Smad7 antibody (aSmad7) and immuno- blotted (IB) with antibodies against TGF- receptor type I (aTGFRI) or aSmad7. These data are representative of three independent experiments. The den- sitometry data are presented as relative density compared with the density at the basal (untreated) condition. **, p 0.01 versus basal expression.

Article Snippet: Anti-Smad2/3 rabbit polyclonal antibody (aSmad2/3), anti-phospho-Ser465/467-specific Smad2/3 rabbit polyclonal antibody (apSmad2/3), anti-Smad7 rabbit polyclonal antibody (aSmad7), and TGF- receptor type I (ALK5) mouse monoclonal antibody (aTGFRI) were obtained from Cell Signaling Technology (Beverly, MA), Santa Cruz Biotechnology (Santa Cruz, CA), Upstate Biotechnology (Lake Placid, NY), and BD Biosciences, respectively.

Techniques: Binding Assay, Incubation, Immunoprecipitation, Expressing